TB-500 is a synthetic peptide based on a naturally occurring protein involved in tissue repair and regeneration. This protocol outlines a daily subcutaneous dosing schedule, gradually increasing from 500 mcg to 1000 mcg over 8-12 weeks, with a recommended reconstitution using 3.0 mL of bacteriostatic water.
- Synthetic peptide fragment of thymosin beta-4.
- Aims to promote tissue repair, wound healing, and blood vessel formation.
- Gradual titration from 500 mcg to 1000 mcg daily.
- Reconstitute with 3.0 mL of bacteriostatic water for easy dosing.
Reconstitution
Vial strength
10 mg
Bacteriostatic water
3 mL
Concentration
3.33 mg/mL
1 unit = 0.01 mL = 33.3 mcg
- 1Draw 3.0 mL of bacteriostatic water using a sterile syringe.
- 2Slowly inject the water down the inner wall of the TB-500 vial, avoiding direct spraying onto the peptide powder to prevent foaming.
- 3Gently swirl or roll the vial until the peptide powder completely dissolves. Do not shake.
- 4Label the reconstituted vial with the date and concentration. Store in the refrigerator at 2-8 °C, away from light.
Dosing schedule
| Phase | Dose | On a U-100 syringe |
|---|---|---|
| Weeks 1-2 | 500 mcg | 15 units (0.15 mL) |
| Weeks 3-4 | 600 mcg | 18 units (0.18 mL) |
| Weeks 5-8 | 750 mcg | 23 units (0.23 mL) |
| Weeks 9-12 | 1000 mcg | 30 units (0.30 mL) |
Protocol detail
| Start | Begin with 500 mcg daily. Increase the dose by approximately 100–150 mcg every two weeks as tolerated. |
|---|---|
| Target | Aim for a daily dose of 750–1000 mcg by Weeks 5–12. |
| Frequency | Inject once per day subcutaneously. |
| Cycle Length | Typically 8–12 weeks; an optional extension to 16 weeks can be considered based on the research protocol. |
| Timing | Inject at a consistent time each day and systematically rotate injection sites. |
Overview at a glance
| Goal | Support tissue repair, wound healing, and angiogenesis through the active thymosin beta-4 fragment mechanism. |
|---|---|
| Schedule | Daily subcutaneous injections for 8–12 weeks; extend to 16 weeks if research objectives require. |
| Dose Range | 500–1000 mcg daily, with gradual titration (~5 mg/week average). |
| Reconstitution | 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements. |
| Storage | Lyophilized frozen; reconstituted refrigerated; avoid freeze–thaw cycles of reconstituted solution. |
How it works
TB-500 is the active N-terminal fragment of thymosin beta-4, specifically the heptapeptide sequence Ac‑LKKTETQ, which is responsible for the full molecule's actin-binding and cell-migration properties. This peptide aids in angiogenesis, wound healing, and tissue regeneration by enhancing cell motility and promoting blood vessel formation, as shown in preclinical studies. Research indicates that TB-500 can boost collagen deposition and reduce healing times in injury sites in animal models. Some metabolic studies suggest it may function as a prodrug, converting into an active pentapeptide metabolite that drives its biological effects.
Reported effects
- Accelerated wound healing and tissue repair
- Enhanced angiogenesis (new blood vessel formation)
- Improved cell migration for recovery
- Potential reduction in inflammation and fibrosis
Reported side effects
- Mild injection-site reactions (redness, tenderness)
- Limited human safety data available
Storage
Lyophilised (unmixed)
Store at -20 °C (-4 °F) in dry, dark conditions; minimize moisture exposure.
After reconstitution
Refrigerate at 2-8 °C (35.6-46.4 °F); do not freeze. Use within 28 days.
Supplies
| Peptide vials | Approximately 4 vials for 8 weeks, 7 for 12 weeks, or 10 for 16 weeks (10 mg each). |
|---|---|
| Insulin Syringes (U‑100) | 7 per week; e.g., 56 for 8 weeks, 84 for 12 weeks, 112 for 16 weeks. |
| Bacteriostatic Water (10 mL bottles) | Recommended 2-3 bottles depending on cycle length (approx. 3.0 mL per 10 mg vial). |
| Alcohol Swabs | Minimum 14 per week (2 per day); e.g., 2 boxes for 8-12 weeks. |
Important notes
- Always use a new, sterile insulin syringe for each injection.
- Rotate injection sites to minimize irritation and lipohypertrophy.
- Inject slowly and allow a few seconds before withdrawing the needle to prevent backflow.
- Document daily doses and injection sites for consistent tracking.
- TB-500 is not FDA-approved for human use and is banned by WADA.
Frequently asked questions
What is the primary function of TB-500?
TB-500 primarily aims to enhance tissue repair and regeneration, accelerate wound healing, and promote angiogenesis (the formation of new blood vessels) by mimicking the active region of thymosin beta-4.
How should I store TB-500 before and after reconstitution?
Lyophilized (powder) TB-500 should be stored frozen at -20 °C (-4 °F). Once reconstituted with bacteriostatic water, it must be refrigerated at 2-8 °C (35.6-46.4 °F) and is typically stable for up to 28 days. Do not freeze the reconstituted solution.
Is TB-500 FDA-approved for human use?
No, TB-500 is not approved by the FDA for human use and is intended for research purposes only. It is also a prohibited substance in competitive sports under WADA regulations.
Why is a gradual increase in dosage recommended for TB-500?
A gradual dosage increase, from 500 mcg up to 1000 mcg daily, allows for assessment of individual response and tolerance, optimizing the protocol while potentially minimizing any mild side effects, consistent with typical research titration practices.
Educational reference only. These figures summarise how TB-500 is commonly described in research literature and supplier documentation, including the TB-500 listing at peptidesuk4u.co.uk. They are not a prescription, not medical advice, and not a recommendation to self-administer anything.
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