Semax is a synthetic heptapeptide derived from ACTH that is typically administered intranasally. It has been researched for its potential neurological and cognitive benefits, including effects on attention and memory. Dosing involves reconstituting the peptide and then using a metered nasal spray to deliver specific microgram (mcg) amounts.
- Intranasal administration is the primary research route.
- Common final volumes are 5 mL or 10 mL for nasal spray bottles.
- A verified 0.10 mL pump output is crucial for accurate dosing.
- Research doses often range from 50 mcg to 400 mcg per administration.
- Priming the nasal pump and consistent technique are important for accuracy.
Reconstitution
Vial strength
5 mg
Bacteriostatic water
3 mL
Concentration
1.67 mg/mL
This is for the initial reconstitution volume of 3.0 mL in the vial. Final concentration depends on total volume in nasal spray bottle.
- 1Confirm the 5 mg vial, selected sterile research vehicle, actuator bottle capacity, and documented pump output.
- 2Add 3.0 mL of compatible sterile vehicle (e.g., sterile 0.9% saline) to the peptide vial using aseptic technique.
- 3Gently swirl the vial until the contents are fully dissolved. Avoid shaking.
- 4Transfer the entire 3.0 mL solution into a sterile 5 mL or 10 mL nasal actuator bottle.
- 5Add additional vehicle to reach a precise final volume of either 5.0 mL or 10.0 mL (approximately 2.0 mL or 7.0 mL more, respectively).
- 6Secure the pump onto the bottle and label it prominently with the peptide amount, final volume, concentration, pump output, and preparation date.
- 7Prime the pump according to the actuator's instructions and record the number of priming sprays in your research log.
Dosing schedule
| Phase | Dose | On a U-100 syringe |
|---|---|---|
| 5 mL Bottle (100 mcg/spray) | 200-500 mcg | 2-5 sprays |
| 10 mL Bottle (50 mcg/spray) | 200-500 mcg | 4-10 sprays |
Protocol detail
| 200 mcg Dose | 2 sprays (5 mL bottle) or 4 sprays (10 mL bottle) |
|---|---|
| 300 mcg Dose | 3 sprays (5 mL bottle) or 6 sprays (10 mL bottle) |
| 400 mcg Dose | 4 sprays (5 mL bottle) or 8 sprays (10 mL bottle) |
| 500 mcg Dose | 5 sprays (5 mL bottle) or 10 sprays (10 mL bottle) |
Overview at a glance
| Human Research Route | Intranasal drops or solution |
|---|---|
| Peptide Amount (Total) | 5,000 mcg |
| 5 mL Option (100 mcg per 0.10 mL spray) | 1,000 mcg/mL |
| 10 mL Option (50 mcg per 0.10 mL spray) | 500 mcg/mL |
| Key Rule | Always reach an exact measured final volume in the nasal bottle. |
How it works
Semax is a synthetic heptapeptide structurally related to ACTH(4-10), but it lacks the hormonal activity of the full ACTH molecule. Its mechanism of action involves influencing neurotrophic signaling pathways, potentially supporting neuronal health and function. Research indicates its involvement in processes related to attention, memory, and various neurological outcomes. When administered intranasally, Semax is believed to be absorbed through the nasal mucosa and interact with brain regions responsible for cognitive and neurological functions, without directly acting as a hormone.
Reported effects
- Improved attention and focus
- Enhanced memory
- Potential neuroprotective effects
- Support for neurological recovery in condition-specific studies
Reported side effects
- Mild nasal mucosal irritation (with prolonged use)
- Variability in delivery due to device and technique
Storage
Lyophilised (unmixed)
Store protected from light and at temperatures no more than 10 degrees C (do not freeze).
After reconstitution
Store protected from light, refrigerated, with the cap secured. Minimize warming and light exposure.
Supplies
| Peptide vials | Semax 5 mg vial |
|---|---|
| Sterile vehicle | Sterile 0.9% sodium chloride or similar |
| Nasal actuator bottle | 5 mL or 10 mL sterile bottle |
| Metered pump | Compatible actuator verified at 0.10 mL/spray |
| Transfer tools | Calibrated sterile syringes/pipettes, sterile transfer needle (if needed) |
| Handling supplies | Alcohol pads, gloves, disinfected work surface |
| Documentation | Labels, research log |
| Safety | Sharps container |
Important notes
- Accurate measurement of final volume, not just bottle capacity, is critical.
- Verify the actuator's output (e.g., 0.10 mL per spray) before calculations.
- Prime the pump as instructed and consistently use full, smooth strokes.
- Keep detailed records of preparation, priming sprays, and actuations.
- This content is for educational purposes only and not medical advice.
Frequently asked questions
Why is it important to verify the nasal spray actuator's output?
Nasal spray pumps can vary significantly in the volume they dispense per spray (e.g., 0.025 mL to 0.20 mL). Verifying the specific output of your pump (often 0.10 mL) is essential for accurately calculating and delivering the intended microgram dose of Semax.
What is the difference between the 5 mL and 10 mL nasal spray bottle options?
Both options utilize the full 5 mg Semax vial but result in different final concentrations. The 5 mL bottle yields a concentration of 1,000 mcg/mL (100 mcg per 0.10 mL spray), while the 10 mL bottle yields 500 mcg/mL (50 mcg per 0.10 mL spray). This means you would use fewer sprays for a given dose with the 5 mL bottle.
Can I use tap water for reconstitution?
No, it is critical to use a sterile research vehicle such as sterile 0.9% sodium chloride. Tap water is not sterile and can introduce contaminants, compromising the integrity and safety of the reconstituted peptide solution.
How does Semax compare to other peptides for cognitive enhancement?
Semax is distinct in its origin as an ACTH(4-10) analog developed for its non-hormonal neurotrophic properties. Unlike some other peptides that might target growth hormone release or gut health, Semax research focuses more directly on attention, memory, and neurological recovery, making it unique within the realm of cognitive-enhancing peptides.
Educational reference only. These figures summarise how Semax is commonly described in research literature and supplier documentation, including the Semax listing at peptidesuk4u.co.uk. They are not a prescription, not medical advice, and not a recommendation to self-administer anything.
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