5-Amino-1MQ is a selective inhibitor of Nicotinamide N-methyltransferase (NNMT) that is being studied for its metabolic properties. Dosing is typically 2.5-5 mg daily via subcutaneous injection, with a 10 mg vial reconstituted to 5 mg/mL.
- Blocks NNMT to support fat metabolism and preserve lean muscle.
- Raises NAD+ levels and activates SIRT1 pathways.
- Typically dosed subcutaneously at 2.5-5 mg per day.
- One 10 mg vial provides 2-4 days of material at standard doses.
- Reconstitute with 2.0 mL of bacteriostatic water for a 5 mg/mL concentration.
Reconstitution
Vial strength
10 mg
Bacteriostatic water
2 mL
Concentration
5 mg/mL
1 unit = 0.01 mL = 50 mcg (0.05 mg)
- 1Remove the vial from freezer storage and allow it to reach room temperature for 15-20 minutes.
- 2Draw 2.0 mL of bacteriostatic water using a sterile syringe.
- 3Slowly inject the water down the inner wall of the vial, avoiding foaming.
- 4Gently swirl or roll the vial until the peptide powder is fully dissolved; the solution should be clear. Do not shake.
- 5Label the reconstituted vial with the date and store it in the refrigerator at 2-8 °C (35.6-46.4 °F), protected from light. Use within 2-4 weeks.
Dosing schedule
| Phase | Dose | On a U-100 syringe |
|---|---|---|
| Days 1-2 (Tolerance) | 2.5 mg | 50 units (0.50 mL) |
| Days 3+ (Standard) | 5 mg | 100 units (1.00 mL) |
| Alternative BID | 2.5 mg twice daily | 50 units (0.50 mL) x 2 |
Protocol detail
| Start | Begin with 2.5 mg once daily to assess individual tolerance. |
|---|---|
| Target | Aim for 5 mg once daily or 2.5 mg twice daily (BID). |
| Frequency | Administer once or twice per day via subcutaneous injection. |
| Vial Duration | A single 10 mg vial will last between 2 to 4 days when adhering to standard doses. |
| Timing | Morning administration is generally preferred; for BID dosing, injections can be split between AM and PM. |
Overview at a glance
| Goal | Support metabolic efficiency through NNMT inhibition, potentially enhancing fat oxidation and NAD+ levels. |
|---|---|
| Schedule | Daily subcutaneous injections; each 10 mg vial provides 2-4 days of material. |
| Dose Range | 2.5–5 mg once or twice daily. |
| Reconstitution | 2.0 mL per 10 mg vial (5 mg/mL) for accurate measurements. |
| Storage | Lyophilized frozen at -20 °C (-4 °F); reconstituted refrigerated at 2-8 °C (35.6-46.4 °F). |
How it works
5-Amino-1MQ is a synthetic small molecule that functions as a selective inhibitor of Nicotinamide N-methyltransferase (NNMT). NNMT is an enzyme that, in conditions like obesity, can be overactive, leading to a depletion of nicotinamide needed for NAD+ synthesis. By inhibiting NNMT, 5-Amino-1MQ helps conserve nicotinamide, promoting NAD+ production through the salvage pathway. This activation of NAD+ then supports SIRT1 pathways, which are crucial for mitochondrial function, fat oxidation, and overall metabolic flexibility. Preclinical studies suggest this inhibition may protect against diet-induced weight gain and improve metabolic health.
Reported effects
- May support reductions in fat mass and preserve lean muscle.
- Associated with elevated NAD+ levels and SIRT1 activation.
- Enhanced grip strength observed in aged mice when combined with exercise.
Reported side effects
- Generally well tolerated.
- Mild headache.
- Transient jitteriness.
- Injection-site reactions.
Storage
Lyophilised (unmixed)
Store frozen at -20 °C (-4 °F) in dry, dark conditions; stable up to 24 months.
After reconstitution
Refrigerate at 2-8 °C (35.6-46.4 °F); use within 2-4 weeks after reconstitution. Do not refreeze.
Supplies
| Peptide vials | 10 mg 5-Amino-1MQ vials (e.g., 4 vials for a 1-week protocol at 5 mg/day). |
|---|---|
| Insulin Syringes | U-100, 1 mL insulin syringes (e.g., 7 for a 1-week once-daily protocol). |
| Bacteriostatic Water | 10 mL bottles of bacteriostatic water (e.g., 1 bottle for a 1-week protocol). |
| Alcohol Swabs | Alcohol swabs for vial stoppers and injection sites (recommend a 100-count box). |
Important notes
- Always use a new, sterile insulin syringe for each injection and dispose of it in a sharps container.
- Rotate injection sites (e.g., abdomen, thighs, upper arms) to minimize local irritation.
- Inject slowly; a mild stinging sensation might be experienced due to the quinolinium structure.
- Keep a record of daily doses and injection site rotation for consistency.
- The 10 mg vial format requires multiple vials for longer protocols, so plan your supplies accordingly.
Frequently asked questions
What is the primary action of 5-Amino-1MQ?
5-Amino-1MQ functions as a selective inhibitor of Nicotinamide N-methyltransferase (NNMT), an enzyme involved in metabolism. By blocking NNMT, it helps increase NAD+ levels, which in turn supports metabolic health and fat oxidation.
How should I store 5-Amino-1MQ?
Lyophilized (powder) 5-Amino-1MQ should be stored frozen at -20 °C (-4°F). Once reconstituted with bacteriostatic water, it must be refrigerated at 2-8 °C (35.6-46.4°F) and used within 2-4 weeks. Do not refreeze the reconstituted solution.
What is the correct way to reconstitute the 10 mg vial?
Allow the vial to reach room temperature, then slowly inject 2.0 mL of bacteriostatic water down the vial wall. Gently swirl or roll until completely dissolved, ensuring not to shake the vial, to achieve a concentration of 5 mg/mL.
Why is 5-Amino-1MQ sometimes dosed twice daily?
Due to its plasma half-life of approximately 3.8-6.9 hours, a twice-daily (BID) dosing schedule may be used to provide more consistent and sustained inhibition of NNMT throughout the day, potentially maximizing its metabolic effects.
Educational reference only. These figures summarise how 5-Amino-1MQ is commonly described in research literature and supplier documentation, including the 5-Amino-1MQ listing at peptidesuk4u.co.uk. They are not a prescription, not medical advice, and not a recommendation to self-administer anything.
Related protocols
5-Amino-1MQ (50 mg Vial)
NNMT inhibitor for metabolic support, fat metabolism, and NAD+ levels.
HGH 191AA (10IU Vial)
Recombinant human growth hormone for body composition and metabolic health.
L-Carnitine (200 mg Vial)
Fuel fat metabolism, enhance energy, bypass gut issues.
MOTS-c (40 mg Vial)
Metabolic regulator enhancing insulin sensitivity and fat oxidation.