Vilon is a synthetic immunoregulatory dipeptide that may enhance immune markers like CD5+ lymphocytes. It is typically administered via subcutaneous injection in a pulsed fashion, including 5 consecutive days of dosing followed by a rest period, repeated monthly. The standard dose range is between 67-667 mcg daily.
- Synthetic dipeptide (Lys-Glu) with immunoregulatory properties.
- Dosed in 5-day pulsed cycles, repeated monthly.
- Reconstituted with 3.0 mL bacteriostatic water for a 6.67 mg/mL concentration.
- Typical daily dose range is 67-667 mcg via subcutaneous injection.
Reconstitution
Vial strength
20 mg
Bacteriostatic water
3 mL
Concentration
6.67 mg/mL
1 unit = 0.01 mL = 66.7 mcg
- 1Draw 3.0 mL of bacteriostatic water with a sterile syringe.
- 2Inject the water slowly down the side of the vial, being careful to avoid foaming.
- 3Gently swirl or roll the vial until the peptide is fully dissolved; do not shake.
- 4Label the reconstituted vial with the date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light. Use within approximately 1 week or aliquot and freeze for extended stability.
Dosing schedule
| Phase | Dose | On a U-100 syringe |
|---|---|---|
| Cycle 1, Day 1 | 67 mcg (0.067 mg) | 1 unit (0.01 mL) |
| Cycle 1, Day 2 | 133 mcg (0.133 mg) | 2 units (0.02 mL) |
| Cycle 1, Day 3 | 200 mcg (0.20 mg) | 3 units (0.03 mL) |
| Cycle 1, Day 4 | 267 mcg (0.267 mg) | 4 units (0.04 mL) |
| Cycle 1, Day 5 | 333 mcg (0.33 mg) | 5 units (0.05 mL) |
| Cycle 2+ (Days 1–5) | 333–667 mcg (0.33–0.67 mg) | 5–10 units (0.05–0.10 mL) |
Protocol detail
| Start | Begin with 67 mcg (1 unit) on Day 1; increase the dose by approximately 67 mcg (1 unit) each day during the first cycle. |
|---|---|
| Target | Aim for a dose of 333–667 mcg (5–10 units) daily from Cycle 2 onwards. |
| Frequency | Administer once per day via subcutaneous injection for 5 consecutive days per cycle. |
| Cycle Length | Each cycle consists of 5 days of injections followed by approximately 23 days off, repeating for 2–4 cycles total. |
| Timing | Maintain a consistent injection time each day and rotate injection sites. |
Overview at a glance
| Goal | Support immune modulation and thymic function based on preclinical observations. |
|---|---|
| Schedule | 5 consecutive days of subcutaneous injections per 4-week cycle; repeat for 2–4 cycles. |
| Dose Range | 67–667 mcg daily with gradual titration during the first cycle. |
| Reconstitution | 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate low-volume measurements. |
| Storage | Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze-thaw cycles. |
How it works
Vilon is a synthetic bioregulatory dipeptide derived from Russian gerontology research. In laboratory settings, the Lys-Glu dipeptide has demonstrated abilities to modulate lymphocyte populations and cytokine gene expression. It has been observed to increase CD5+ cell counts in thymus cell cultures and elevate interleukin-2 (IL-2) mRNA expression in splenocyte preparations. Additional preclinical studies suggest potential effects on TGF-β1 and vascular permeability in kidney models. The pulsed 5-day monthly regimen is inspired by similar short peptide protocols.
Reported effects
- Supports immune cell marker expression (CD5+ lymphocytes) in cell cultures.
- Increases IL-2 gene activity in cell culture models.
- May modulate inflammatory cytokines like TGF-β1 under experimental renal stress.
- Early animal studies indicate potential for tumor growth inhibition and lifespan extension.
Reported side effects
- Generally well-tolerated in preclinical settings.
- Occasional mild injection-site reactions (e.g., redness, itching) from subcutaneous administration.
Storage
Lyophilised (unmixed)
Store at -20 °C (-4 °F) short-term or -80 °C (-112 °F) long-term in dry, dark conditions; minimize moisture. Allow to reach room temperature before opening.
After reconstitution
Refrigerate at 2-8 °C (35.6-46.4 °F) and use within ~1 week. For longer storage, aliquot and freeze.
Supplies
| Peptide vials (Vilon, 20 mg each) | 1 vial for an 8-16 week protocol (10-20 injections). |
|---|---|
| Insulin Syringes (U-100, 30- or 50-unit preferred) | 5 syringes per cycle, requiring 10-20 syringes for an 8-16 week protocol. |
| Bacteriostatic Water (10 mL bottles) | One 10 mL bottle for reconstituting one vial. |
| Alcohol Swabs | Ten swabs per cycle (two per day), requiring 20-40 swabs (recommend one 100-count box) for an 8-16 week protocol. |
Important notes
- Always use new, sterile insulin syringes (30- or 50-unit recommended for accuracy) and dispose of them in a sharps container.
- Rotate injection sites (e.g., abdomen, thighs, upper arms) to minimize local irritation.
- Inject slowly and allow a few seconds before withdrawing the needle.
- Maintain a detailed record of daily doses and injection site rotation to ensure consistency throughout the cycles.
Frequently asked questions
What is Vilon and how is it different from other peptides?
Vilon is a synthetic immunoregulatory dipeptide composed of lysine and glutamic acid. Unlike some longer peptides, it's known for its specific immune-modulating properties, especially regarding CD5+ lymphocytes and IL-2 expression, based on preclinical research. Its dosing schedule is unique, following a pulsed 5-day cycle.
Can I adjust the Vilon dosage for faster results?
The provided protocol outlines a gradual titration during the first cycle and a maintained dose thereafter. It's crucial not to deviate from established protocols, as altering dosages without scientific backing can lead to unpredictable outcomes or reduced efficacy. Always adhere to the recommended ranges for research purposes.
How important is the rest period between Vilon cycles?
The rest period of approximately 23 days between 5-day injection phases is integral to the pulsed protocol. This cycle structure is designed to mimic natural biological rhythms or optimize the peptide's effects, potentially preventing desensitization or allowing the immune system to respond optimally. Adhering to the full cycle length is important for evaluating results.
What's the best way to ensure accuracy with such small doses?
For accurate measurement of Vilon, it's recommended to reconstitute the 20mg vial with 3.0 mL of bacteriostatic water, yielding a concentration of approximately 6.67 mg/mL. Using 30- or 50-unit U-100 insulin syringes is crucial, as their fine gradations (1 unit corresponds to ~66.7 mcg) allow for precise administration of the small required volumes. Always double-check your calculations.
Educational reference only. These figures summarise how Vilon is commonly described in research literature and supplier documentation, including the Vilon listing at peptidesuk4u.co.uk. They are not a prescription, not medical advice, and not a recommendation to self-administer anything.
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