5-Amino-1MQ (50 mg Vial) Dosage Protocol

Also known as: 5-amino-1-methylquinolinium

NNMT inhibitor for metabolic support, fat metabolism, and NAD+ levels.

Dose range
2.5-5 mg daily
Route
Subcutaneous
Frequency
Once or twice daily
Cycle length
10-20 days per 50 mg vial

5-Amino-1MQ is a selective inhibitor of Nicotinamide N-methyltransferase (NNMT), an enzyme implicated in metabolic dysfunction. This peptide is studied for its potential to improve fat metabolism and increase NAD+ levels. Dosing typically ranges from 2.5mg to 5mg daily via subcutaneous injection.

  • Selective NNMT inhibitor.
  • Supports fat metabolism and NAD+ levels.
  • Dosed subcutaneously at 2.5-5 mg daily.
  • One 50 mg vial provides 10-20 days of material.
  • Reconstitute with 4.0 mL bacteriostatic water.

Reconstitution

Vial strength

50 mg

Bacteriostatic water

4 mL

Concentration

12.5 mg/mL

1 unit = 0.01 mL = 0.125 mg (125 mcg)

  1. 1Remove the vial from freezer storage and allow it to reach room temperature for 15-20 minutes.
  2. 2Draw 4.0 mL of bacteriostatic water with a sterile syringe.
  3. 3Slowly inject the water down the inner wall of the vial, avoiding direct contact with the peptide powder to prevent foaming.
  4. 4Gently swirl or roll the vial until the powder is completely dissolved and the solution is clear; do not shake.
  5. 5Label the reconstituted vial with the date and refrigerate at 2-8 °C (35.6-46.4 °F), protected from light. Use within 2-4 weeks.

Dosing schedule

PhaseDoseOn a U-100 syringe
Days 1-2 (Tolerance)2.5 mg once daily20 units (0.20 mL)
Days 3+ (Standard)5 mg once daily40 units (0.40 mL)
Alternative BID2.5 mg twice daily20 units (0.20 mL) x 2

Protocol detail

StartBegin with 2.5 mg once daily to assess individual tolerance.
TargetIncrease to 5 mg once daily or opt for 2.5 mg twice daily (BID).
FrequencyAdminister once or twice per day via subcutaneous injection.
Vial DurationA single 50 mg vial typically lasts 10–20 days at recommended research doses.
TimingMorning administration is generally preferred; twice-daily doses can be split between morning and evening.

Overview at a glance

GoalTo support metabolic efficiency by inhibiting NNMT, potentially enhancing fat oxidation and boosting NAD+ levels.
ScheduleDaily subcutaneous injections, with each 50 mg vial supplying 10-20 days of material.
Dose Range2.5–5 mg once or twice daily.
ReconstitutionUse 4.0 mL of bacteriostatic water per 50 mg vial to achieve a concentration of 12.5 mg/mL for precise measurements.
StorageLyophilized vials should be frozen at -20 °C (-4 °F); once reconstituted, refrigerate at 2-8 °C (35.6-46.4 °F).

How it works

5-Amino-1MQ is a synthetic small molecule that acts as a selective inhibitor of Nicotinamide N-methyltransferase (NNMT). This enzyme is often overexpressed in adipose tissue in conditions of obesity and metabolic dysfunction, leading to the depletion of nicotinamide and reduced availability of NAD+. By blocking NNMT, 5-Amino-1MQ helps conserve nicotinamide, allowing for increased NAD+ synthesis. This action can then activate SIRT1 pathways, which are crucial for mitochondrial biogenesis and fat oxidation. Preclinical studies suggest that NNMT inhibition can mitigate diet-induced weight gain and improve metabolic health without altering food intake.

Reported effects

  • May support reductions in fat mass.
  • Helps preserve lean muscle mass.
  • Associated with elevated NAD+ levels.
  • Activates SIRT1 pathways.
  • Potential for improved metabolic parameters.

Reported side effects

  • Generally well tolerated.
  • Mild headache.
  • Transient jitteriness.
  • Injection-site reactions.
  • Long-term human safety data is not established.

Storage

Lyophilised (unmixed)

Store at -20 °C (-4 °F) in dry, dark conditions; stable up to 24 months.

After reconstitution

Refrigerate at 2-8 °C (35.6-46.4 °F); use within 2-4 weeks. Do not refreeze.

Supplies

Peptide vials5-Amino-1MQ 50 mg vials (1 vial for 1-2 weeks, 2 vials for 4 weeks at 5mg/day)
Insulin SyringesU-100 type (7 per week for once daily dosing, 14 per week for BID dosing)
Bacteriostatic Water10 mL bottles (4.0 mL needed per 50 mg vial)
Alcohol SwabsOne for vial stopper, one for injection site per administration (approx. 14 per week for once daily dosing)

Important notes

  • Always use a new, sterile insulin syringe for each administration and dispose of it properly in a sharps container.
  • Rotate injection sites frequently (e.g., abdomen, thighs, upper arms) to prevent local irritation and lipohypertrophy.
  • Inject slowly; a slight stinging sensation may be experienced due to the compound's quinolinium structure.
  • Maintain a record of your daily doses and injection site rotation for consistency and accurate tracking.
  • This product is for research use only and not for human consumption.

Frequently asked questions

What is the recommended reconstitution for a 50 mg vial of 5-Amino-1MQ?

For a 50 mg vial, you should add 4.0 mL of bacteriostatic water. This will yield a concentration of 12.5 mg/mL, making it easier to measure doses accurately using an insulin syringe.

How long will a 50 mg vial of 5-Amino-1MQ last?

At typical research doses of 2.5-5 mg per day, a single 50 mg vial will generally last between 10 to 20 days, depending on your daily dosing schedule (once or twice daily).

What is the primary mechanism of action for 5-Amino-1MQ?

5-Amino-1MQ works by selectively inhibiting the enzyme Nicotinamide N-methyltransferase (NNMT). This inhibition helps prevent the breakdown of nicotinamide, leading to increased NAD+ levels and activation of SIRT1 pathways, which are crucial for cellular metabolism and fat oxidation.

What are the common storage guidelines for 5-Amino-1MQ?

Lyophilized (powder) vials should be stored frozen at -20 °C (-4 °F). Once reconstituted with bacteriostatic water, the solution should be refrigerated at 2-8 °C (35.6-46.4 °F) and used within 2-4 weeks. Do not refreeze the reconstituted solution.

Educational reference only. These figures summarise how 5-Amino-1MQ is commonly described in research literature and supplier documentation, including the 5-Amino-1MQ listing at peptidesuk4u.co.uk. They are not a prescription, not medical advice, and not a recommendation to self-administer anything.

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