Sermorelin (10 mg Vial) Dosage Protocol

Stimulates natural growth hormone, supports IGF-1 levels.

Dose range
200-500 mcg daily
Route
Subcutaneous
Frequency
Once daily
Cycle length
8-12 weeks

Sermorelin is a synthetic peptide that stimulates the body's natural growth hormone release. It is typically administered via subcutaneous injection once daily at bedtime, with dosages ranging from 200-500 mcg. This protocol is designed to align with the body's natural nocturnal growth hormone pulse.

  • Synthetic GHRH analog that stimulates natural GH release.
  • Administered once daily at bedtime via subcutaneous injection.
  • Dosage typically ranges from 200-500 mcg daily.
  • Commonly used to support IGF-1 levels and anabolism.
  • Avoids excessive GH levels due to preserved feedback loops.

Reconstitution

Vial strength

10 mg

Bacteriostatic water

3 mL

Concentration

3.33 mg/mL

1 unit on a U-100 syringe = 0.01 mL = 33.3 mcg

  1. 1Draw 3.0 mL of bacteriostatic water with a sterile syringe.
  2. 2Inject the water slowly down the side of the vial; gently swirl the vial until the powder completely dissolves (do not shake vigorously).
  3. 3Label the vial with the reconstitution date and refrigerate it at 2–8 °C (36–46 °F), protected from light.
  4. 4Before each use, wipe the vial stopper with an alcohol swab and use a new sterile needle and syringe for each injection.

Dosing schedule

PhaseDoseOn a U-100 syringe
Weeks 1-2200 µg6 units (0.06 mL)
Weeks 3-4300 µg9 units (0.09 mL)
Weeks 5-6400 µg12 units (0.12 mL)
Weeks 7-8500 µg15 units (0.15 mL)

Protocol detail

StartBegin with 200 µg nightly at bedtime, and gradually increase by approximately 100 µg every 1–2 weeks as tolerated.
TargetAim for a dose of 300–500 µg nightly by Weeks 5–8, adjusting based on individual IGF-1 responses.
FrequencyOnce daily, administered subcutaneously, preferably before sleep.
Cycle LengthTypically 3–6 months for adult research use.
TimingBedtime administration is crucial to leverage the body's natural nocturnal GH pulse. Rotate injection sites systematically.

Overview at a glance

GoalTo stimulate the pituitary's natural growth hormone (GH) release, thereby supporting physiological IGF-1 levels and anabolic processes.
ScheduleDaily subcutaneous injections at bedtime, typically for 3–6 months.
Dose Range200–500 µg daily (adult research range).
Reconstitution3.0 mL of bacteriostatic water per 10 mg vial, resulting in approximately 3.33 mg/mL concentration.
StorageLyophilized vials are refrigerated; reconstituted solution is refrigerated and used within 10–14 days.

How it works

Sermorelin, a synthetic analog of growth hormone-releasing hormone (GHRH), activates GHRH receptors on pituitary somatotropes, leading to the body's own pulsatile GH secretion. Unlike direct GH administration, Sermorelin maintains the body's natural regulatory mechanisms, such as negative feedback from somatostatin and IGF-1. This helps prevent excessive GH or IGF-1 levels. The resulting pulsatile GH output stimulates IGF-1 production in the liver and other tissues, promoting protein synthesis, fat breakdown, and tissue repair. This approach aims to restore GH output, especially in adults experiencing age-related declines, by supporting physiological processes.

Reported effects

  • Stimulates endogenous pulsatile GH release.
  • Supports physiological IGF-1 elevation.
  • May improve body composition and energy (adults, off-label).
  • Preserves natural feedback mechanisms, reducing supraphysiological GH risk.

Reported side effects

  • Injection-site reactions (redness, pain, swelling) are common.
  • Rare systemic effects include headache, flushing, dizziness, or hives.
  • Approximately 6.5% of users may develop subclinical hypothyroidism.
  • No serious acromegaly or excessive IGF-1 elevations at recommended doses.

Storage

Lyophilised (unmixed)

Refrigerate at 2–8 °C (36–46 °F) in dry, dark conditions.

After reconstitution

Refrigerate at 2–8 °C (36–46 °F) and use within 10–14 days. Do not freeze.

Supplies

Peptide vials2 vials (10 mg each) for an 8-week protocol, 4 vials for a 12-week protocol.
Insulin Syringes (U-100)Approximately 56 syringes for an 8-week protocol, 84 for 12 weeks. Use 0.3–0.5 mL size with 28G–31G needle, 5/16″ to 1/2″ length. 30- or 50-unit syringes recommended for low doses.
Bacteriostatic WaterOne 10 mL bottle for an 8-week protocol, two 10 mL bottles for a 12-week protocol (uses ~3.0 mL per vial).
Alcohol SwabsTwo 100-count boxes for an 8-week protocol, two for a 12-week protocol (2 per day).
Sharps ContainerOne puncture-proof container sufficient for all used needles.

Important notes

  • Bedtime administration is essential to align with natural nocturnal GH release.
  • For precise low-dose measurements, consider using 30- or 50-unit insulin syringes during early weeks.
  • Always use a new sterile insulin syringe for each injection and dispose of it in a sharps container.
  • Rotate injection sites regularly to prevent skin irritation or tissue changes.
  • Monitor IGF-1 levels periodically (every 1-2 months) and check thyroid function for long-term use.

Frequently asked questions

Why is Sermorelin best injected at bedtime?

Sermorelin stimulates the body's natural growth hormone (GH) release. Injecting it at bedtime is recommended because it aligns with the body's natural nocturnal GH pulse, maximizing its effectiveness and mimicking physiological rhythms.

Can I shake the vial to mix the powder with water?

No, you should not shake the vial vigorously when reconstituting Sermorelin. Instead, inject the bacteriostatic water slowly down the vial wall and gently swirl the vial until the powder is fully dissolved. Shaking can damage the peptide.

What should I do if I experience redness or tenderness at the injection site?

Mild redness, pain, or swelling at the injection site is a common side effect. To minimize this, ensure you are rotating injection sites systematically and injecting into a fatty tissue area. If a site becomes irritated, allow it to rest longer before reusing it.

How long can reconstituted Sermorelin be stored?

Reconstituted Sermorelin, when mixed with bacteriostatic water, should be stored in the refrigerator at 2–8 °C (36–46 °F) and used within 10–14 days. It is important not to freeze the mixed solution.

Educational reference only. These figures summarise how Sermorelin is commonly described in research literature and supplier documentation, including the Sermorelin listing at peptidesuk4u.co.uk. They are not a prescription, not medical advice, and not a recommendation to self-administer anything.

Related protocols

Back to all protocols