Epitalon is a synthetic tetrapeptide derived from the pineal gland, often explored for its potential anti-aging and longevity benefits. This protocol details a daily subcutaneous injection regimen for 20 days, followed by an extended break.
- Activates telomerase for telomere elongation.
- Supports healthy melatonin production and circadian regulation.
- Administered daily via subcutaneous injection for 20 days.
- Generally well-tolerated with no serious side effects reported in studies.
- Protocol involves 4-6 months off-cycle before repeating.
Reconstitution
Vial strength
50 mg
Bacteriostatic water
3 mL
Concentration
16.67 mg/mL
1 unit = 0.01 mL = 166.7 mcg (U-100 syringe)
- 1Draw 3.0 mL of bacteriostatic water with a sterile syringe.
- 2Inject the water slowly down the side of the vial wall, avoiding foaming.
- 3Gently swirl or roll the vial until the powder is fully dissolved; do not shake vigorously.
- 4Label the reconstituted vial with the date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Dosing schedule
| Phase | Dose | On a U-100 syringe |
|---|---|---|
| Days 1-20 (Cycle On) | 5,000 mcg (5 mg) | 100 units (1.00 mL) |
| Weeks 4-26 (Cycle Off) | 0 mcg | — |
Protocol detail
| Standard Dose | 5,000 mcg (5 mg) once daily subcutaneously. |
|---|---|
| Alternative | 10 mg/day for 10 days (same total cycle dose of 100 mg). |
| Frequency | Once per day (subcutaneous), preferably at bedtime. |
| Cycle Length | 20 consecutive days on, then 4–6 months off. |
| Timing | Evening administration recommended; rotate injection sites daily. |
Overview at a glance
| Goal | Support telomere maintenance, melatonin regulation, and geroprotective effects over time. |
|---|---|
| Schedule | Daily subcutaneous injections for 20 consecutive days, followed by 4–6 months off-cycle. |
| Dose | 5,000 mcg (5 mg) daily. |
| Reconstitution | 3.0 mL per 50 mg vial (16.67 mg/mL) for accurate unit measurements. |
| Storage | Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw. |
How it works
Epitalon primarily functions by activating telomerase, an enzyme that promotes the lengthening of telomeres, which are protective caps on the ends of chromosomes. Research indicates this allows cells to divide more times than typically possible. Additionally, Epitalon influences the neuroendocrine system, stimulating nocturnal melatonin release from the pineal gland. This action helps to normalize circadian rhythms, particularly in older individuals. Other reported effects include antioxidant properties, gene regulation, and immune system modulation.
Reported effects
- Supports telomere maintenance and cellular longevity.
- Restores healthy melatonin production and circadian rhythm.
- In animal models, extended lifespan and reduced tumor incidence.
- Human trials suggest improved cardiovascular outcomes and reduced mortality.
Reported side effects
- Generally well tolerated with no serious adverse events reported.
- Occasional mild injection-site reactions.
- Transient changes in sleep patterns might occur.
Storage
Lyophilised (unmixed)
Store long-term at -20 °C (-4 °F), or routinely at 2-8 °C (35.6-46.4 °F); minimize moisture exposure.
After reconstitution
Refrigerate at 2-8 °C (35.6-46.4 °F); use within 2-4 weeks and avoid freeze-thaw cycles.
Supplies
| Peptide vials | 10 Epitalon (10 mg) vials for a 20-day cycle (each vial yields 2 doses). |
|---|---|
| Insulin Syringes (U-100) | 20 syringes per cycle (1 per day). |
| Bacteriostatic Water | 2 x 10 mL bottles for a 20-day cycle (using 3.0 mL per vial). |
| Alcohol Swabs | Approximately 40 swabs per cycle (two per day); a 100-count box is recommended. |
Important notes
- Always use new, sterile insulin syringes and dispose of them in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to minimize local irritation.
- Inject slowly and wait a few seconds before withdrawing the needle.
- Document daily doses and site rotations for consistency.
- Reconstitute one vial at a time (approximately every 2 days) to maintain freshness.
Frequently asked questions
How often should I take Epitalon?
Epitalon is typically dosed once daily for 20 consecutive days. After this 20-day 'on' period, you should observe a 4-6 month 'off' period before considering another cycle.
Why is Epitalon recommended to be injected at night?
Administering Epitalon in the evening or at bedtime is suggested to align with and potentially enhance the body's natural melatonin rhythms, which it helps to regulate, thereby supporting improved sleep and circadian function.
What is the primary benefit of Epitalon?
Epitalon's primary benefit is its potential for anti-aging and longevity support. It achieves this mainly by activating telomerase, an enzyme that helps maintain and lengthen telomeres, which are vital for cellular health and preventing cellular aging.
How do I ensure I'm injecting the correct amount?
After reconstituting a 10 mg Epitalon vial with 3.0 mL of bacteriostatic water, the solution will have a concentration of 16.67 mg/mL. This means 1 unit on a U-100 insulin syringe (0.01 mL) equals 50 mcg. For a 5 mg dose, you would inject 100 units (1.00 mL).
Educational reference only. These figures summarise how Epitalon is commonly described in research literature and supplier documentation, including the Epitalon listing at peptidesuk4u.co.uk. They are not a prescription, not medical advice, and not a recommendation to self-administer anything.
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